An Anaerobic Titration Assembly for Spectrophotometric Use 1

Much useful information of a quantitative nature can be obtained with redox-active proteins by anaerobic titration with reducing substrates or chemical reductants such as sodium dithionite. We have used successfully for many years anaerobic spectrophotometer cells equipped with either two or four side arms from which known amounts of titrant can be added (1). This technique gives only a limited amount of information for a given quantity of enzyme. The need is obvious for a sensitive and versatile apparatus with which a large number of experimental points may be obtained with a single solution of enzyme. Such a titration apparatus is described in this communication.